Lineweaver-Burk plot, also known as a double-reciprocal plot or Eadie-Hofstee plot, is a graphical representation of the Michaelis-Menten equation, which describes the relationship between the reaction rate of an enzyme-catalyzed reaction and the substrate concentration.
The plot is constructed by plotting the reciprocal of the reaction rate (1/v) against the reciprocal of the substrate concentration (1/[S]). The resulting graph is a straight line with a slope of -Km/Vmax and a y-intercept of 1/Vmax. Enzyme kinetics and inhibition kinetics parameters can be derived from this plot.